Improvement in growth performance and digestive function from amniotic injections of N‐acetylglutamate in broiler chickens

Abstract

AbstractBACKGROUNDN‐acetylglutamate (NAG) is the initial and essectial substrate in the process of de novo arginine synthesis, plays an important role in intestinal development. The aim of this study was to determine the effects of in ovo feeding of NAG, 1.5 mg/egg at 17.5 days of incubation (DOI) via amnion, on hatching performance, early intestinal histomorphometry, jejunal barrier, digestive function, and growth performance of broiler chickens between 1 and 14 days of age.RESULTSAmniotic injection of NAG had no significant effect on hatching characteristics compared with the non‐injected control group (NC group). Birds in the NAG solution‐injected group (NAG group) exhibited lower average daily feed intake and better feed efficiency during a period of 1–14 days. In comparison with the NC group, the NAG group had decreased crypt depth (CD) in the ileum and increased villus height (VH) / CD in the jejunum at 7 days, and decreased CD in duodenum and significantly increased VH in the jejunum at 14 days. However, the effects of in ovo supplementation with NAG on the density of goblet cells, and gene expression of mucin 2 and alkaline phosphatase were not significant. Chicks in the NAG group had a significantly higher mRNA expression level of trypsin and maltase in jejunum at 7 days than the NC group but not at 14 days.CONCLUSIONAmniotic injections of NAG (1.5 mg/egg) at 17.5 DOI could improve early growth performance of broilers during 1–14 days after hatching by accelerating the development of the intestine and enhancing jejunal digestive function. © 2023 Society of Chemical Industry.