Affiliation:
1. Department of Reagent Research and Development Shenzhen YHLO Biotech Co., Ltd. Shenzhen Guangdong China
2. Hunan Key Laboratory of Biomedical Nanomaterials and Devices College of Life Science and Chemistry, Hunan University of Technology Zhuzhou Hunan China
3. Department of Biomedical Engineering The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics‐Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Huazhong University of Science and Technology Wuhan Hubei China
Abstract
AbstractRespiratory tract infections are associated with the most common diseases transmitted among people and remain a huge threat to global public health. Rapid and sensitive diagnosis of causative agents is critical for timely treatment and disease control. Here, we developed a novel method based on recombinase polymerase amplification (RPA) combined with CRISPR‐Cas12a to detect three viral pathogens, including SARS‐CoV‐2, influenza A, and influenza B, which cause similar symptom complexes of flu cold in the respiratory tract. The detection method can be completed within 1 h, which is faster than other standard detection methods, and the limit of detection is approximately 102 copies/μL. Additionally, this detection system is highly specific and there is no cross‐reactivity with other common respiratory tract pathogens. Based on this assay, we further developed a more simplified RPA/CRISPR‐Cas12a system combined with lateral flow assay on a manual microfluidic chip, which can simultaneously detect these three viruses. This low‐cost detection system is rapid and sensitive, which could be applied in the field and resource‐limited areas without bulky and expensive instruments, providing powerful tools for the point‐of‐care diagnostic.
Subject
Infectious Diseases,Virology
Cited by
2 articles.
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