Using LanM Enzymes to Modify Glucagon‐Like Peptides 1 and 2 in E.coli

Author:

Larsen Camilla K.12,Lindquist Peter3ORCID,Rosenkilde Mette3ORCID,Madsen Alice R.2,Haselmann Kim2,Glendorf Tine2,Olesen Kjeld2,Kodal Anne Louise B.2,Tørring Thomas1ORCID

Affiliation:

1. Department of Biological and Chemical Engineering Aarhus University 8000 Aarhus C Denmark

2. Novo Nordisk A/S 2760 Måløv Denmark

3. Department of Biomedical Sciences University of Copenhagen 2100 Copenhagen Denmark

Abstract

AbstractSelective modification of peptides is often exploited to improve pharmaceutically relevant properties of bioactive peptides like stability, circulation time, and potency. In Nature, natural products belonging to the class of ribosomally synthesized and post‐translationally modified peptides (RiPPs) are known to install a number of highly attractive modifications with high selectivity. These modifications are installed by enzymes guided to the peptide by corresponding leader peptides that are removed as the last step of biosynthesis. Here, we exploit leader peptides and their matching enzymes to investigate the installation of D‐Ala post‐translationally in a critical position in the hormones, glucagon‐like peptides (GLP) 1 and 2. We also offer insight into how precursor peptide design can modulate the modification pattern achieved.

Publisher

Wiley

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3