Physicochemical, in vitro and in vivo evaluation of VEGF loaded PCL‐mPEG and PDGF loaded PCL‐Chitosan dual layered vascular grafts

Author:

Sultana Tamanna1,Fahad Md. Abdullah Al1ORCID,Park Myeongki1,Kwon Soon Ha2,Lee Byong‐Taek13ORCID

Affiliation:

1. Department of Regenerative Medicine, College of Medicine Soonchunhyang University Cheonan Republic of Korea

2. Department of Surgery Soonchunhyang University Cheonan Hospital Cheonan South Korea

3. Institute of Tissue Regeneration, College of Medicine Soonchunhyang University Cheonan Republic of Korea

Abstract

AbstractThe present study has attempted to evaluate the endothelialization and smooth muscle regeneration efficiency of a novel dual‐layer small‐diameter vascular graft. Two types of layers (PCL‐mPEG‐VEGF and PCL‐Chitosan‐PDGF) were fabricated to find out the best layer giving endothelialization support for the lumen and unique contractile function for outer layer of blood vessels. Platelet‐derived growth factor (PDGF) and chitosan were immobilized onto PCL surface by aminolysis‐based surface modification technique. Besides, Poly (ethylene glycol) methyl ether (mPEG) and vascular endothelial growth factor (VEGF) were directly blended with PCL. Morphological analysis of membranes ensured consistency of average fibers diameter with native extracellular matrix. A favorable interaction of PCL‐mPEG‐VEGF with cow pulmonary endothelial cells (CPAEs) and PCL‐Chitosan‐PDGF with rat bone marrow mesenchymal stem cells (RBMSCs) was obtained during in vitro study. Controlled growth factor release patterns were found from both layers. Further, PCL‐mPEG‐VEGF exhibited endothelial markers expression properties from RBMSCs. Up‐regulation of SMCs markers expression was significantly ensured by the PCL‐Chitosan‐PDGF membrane. Thus, PCL‐mPEG‐VEGF and PCL‐Chitosan‐PDGF were preferred as inner and outer layers respectively of a finally prepared tubular hybrid tissue engineered small diameter vascular graft. Finally, the dual‐layer vascular graft was implanted onto a rat abdominal aorta model for 2 months. The extracted samples exhibited the presence of endothelial marker (ICAM 1) in the inner layer and smooth muscle cell marker (αSMA) in the outer layer as well as substantial amount of collagen deposition was observed in the both layers.

Funder

National Research Foundation of Korea

Ministry of Education

Publisher

Wiley

Subject

Biomedical Engineering,Biomaterials

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3