Bioinformatics approach combined with experimental verification reveals OAS3 gene implicated in paclitaxel resistance in head and neck cancer

Abstract

AbstractBackgroundThis study aimed to identify a candidate gene associated with paclitaxel (PTX) resistance and to evaluate functionally its biological role in the PTX‐resistant head and neck squamous cell carcinoma (HNSCC) cell lines and clinical specimens.MethodsMicroarray data series containing samples of different types of cancers resistant to PTX were analyzed and then a candidate gene associated with PTX resistance was identified using various bioinformatics tools. After the suppression of the target gene expression, changes in cell viability and colony‐forming ability were evaluated in PTX‐resistant FaDu and SCC‐9 cell lines.ResultsBioinformatics analyses of upregulated genes in PTX‐resistant cancer cells indicated that OAS3 was associated with PTX resistance. The downregulation of OAS3 expression significantly reduced the viability and colony‐forming capacity of PTX‐resistant SCC‐9 cells by inducing apoptosis and cell cycle arrest at G0/G1 phase.ConclusionsThe therapeutic targeting of OAS3 may resensitize PTX‐resistant HNSCC cells with high OAS3 expression to PTX treatment.