Next‐Generation Nanopore Sensors Based on Conductive Pulse Sensing for Enhanced Detection of Nanoparticles

Author:

Confederat Samuel12,Lee Seungheon3ORCID,Vang Der4,Soulias Dimitrios125,Marcuccio Fabio126,Peace Timotheus I.127,Edwards Martin Andrew8,Strobbia Pietro4,Samanta Devleena3ORCID,Wälti Christoph12,Actis Paolo12ORCID

Affiliation:

1. Bragg Centre for Materials Research University of Leeds LS2 9JT Leeds UK

2. School of Electronic and Electrical Engineering and Pollard Institute University of Leeds LS2 9JT Leeds UK

3. Department of Chemistry The University of Texas at Austin Austin TX 78712 USA

4. Department of Chemistry University of Cincinnati Cincinnati OH 45221 USA

5. Physical and Theoretical Chemistry Laboratory Department of Chemistry University of Oxford OX1 3QZ Oxford UK

6. Faculty of Medicine Imperial College London SW7 2AZ London UK

7. School of Molecular and Cellular Biology and Astbury Centre for Structural Molecular Biology University of Leeds LS2 9JT Leeds UK

8. Department of Chemistry and Biochemistry University of Arkansas Fayetteville AR 72701 USA

Abstract

AbstractNanopore sensing has been successfully used to characterize biological molecules with single‐molecule resolution based on the resistive pulse sensing approach. However, its use in nanoparticle characterization has been constrained by the need to tailor the nanopore aperture size to the size of the analyte, precluding the analysis of heterogeneous samples. Additionally, nanopore sensors often require the use of high salt concentrations to improve the signal‐to‐noise ratio, which further limits their ability to study a wide range of nanoparticles that are unstable at high ionic strength. Here, a new paradigm in nanopore research that takes advantage of a polymer electrolyte system to comprise a conductive pulse sensing approach is presented. A finite element model is developed to explain the conductive pulse signals observed and compare these results with experiments. This system enables the analytical characterization of heterogeneous nanoparticle mixtures at low ionic strength . Furthermore, the wide applicability of the method is demonstrated by characterizing metallic nanospheres of varied sizes, plasmonic nanostars with various degrees of branching, and protein‐based spherical nucleic acids with different oligonucleotide loadings. This system will complement the toolbox of nanomaterials characterization techniques to enable real‐time optimization workflow for engineering a wide range of nanomaterials.

Funder

Horizon 2020 Framework Programme

European Research Council

Engineering and Physical Sciences Research Council

Medical Research Council

Publisher

Wiley

Subject

Biomaterials,Biotechnology,General Materials Science,General Chemistry

Cited by 8 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3