A novel Bxb1 integrase RMCE system for high fidelity site-specific integration of mAb expression cassette in CHO Cells-Reference-Cited by-同舟云学术

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A novel Bxb1 integrase RMCE system for high fidelity site-specific integration of mAb expression cassette in CHO Cells

Published:2017-03-14 Issue:8 Volume:114 Page:1837-1846
ISSN:0006-3592
Container-title:Biotechnology and Bioengineering
language:en
Short-container-title:Biotechnol. Bioeng.

Author:

Inniss Mara C.¹^ORCID,Bandara Kalpanie¹,Jusiak Barbara²,Lu Timothy K.²,Weiss Ron²,Wroblewska Liliana³,Zhang Lin¹

Affiliation:

1. Cell Line Development; Biotherapeutics Pharmaceutical Science; Pfizer Inc; Andover 01810 Massachusetts

2. Synthetic Biology Center; Department of Biological Engineering; Massachusetts Institute of Technology; Cambridge Massachusetts

3. BioMedicine Design; Pfizer Inc; Cambridge Massachusetts

Publisher

Wiley

Subject

Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Link

http://onlinelibrary.wiley.com/wol1/doi/10.1002/bit.26268/fullpdf

Reference40 articles.

1. CasFinder: Flexible algorithm for identifying specific Cas9 targets in genomes;Aach;bioRxiv,2014

2. Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice;Branda;Dev Cell,2004

3. Site-directed genome modification: Derivatives of DNA-modifying enzymes as targeting tools;Coates;Trends Biotechnol,2005

4. Fast identification of reliable hosts for targeted cell line development from a limited-genome screening using combined phiC31 integrase and CRE-Lox technologies;Crawford;Biotechnol Prog,2013

5. Inducible in vivo genome editing with CRISPR-Cas9;Dow;Nat Biotechnol,2015

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1. The new frontier in CHO cell line development: From random to targeted transgene integration technologies;Biotechnology Advances;2024-10

2. CHO cell engineering via targeted integration of circular miR-21 decoy using CRISPR/RMCE hybrid system;Applied Microbiology and Biotechnology;2024-08-09

3. Increased stable integration efficiency in CHO cells through enhanced nuclear localization of Bxb1 serine integrase;BMC Biotechnology;2024-06-26

4. Systematic Development of Reprogrammed Modular Integrases Enables Precise Genomic Integration of Large DNA Sequences;2024-05-10

5. Large T antigen mediated target gene replication improves site-specific recombination efficiency;Frontiers in Bioengineering and Biotechnology;2024-04-26

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