Clarifying the Difference between Iterative Saturation Mutagenesis as a Rational Guide in Directed Evolution and OmniChange as a Gene Mutagenesis Technique-Reference-Cited by-同舟云学术

Clarifying the Difference between Iterative Saturation Mutagenesis as a Rational Guide in Directed Evolution and OmniChange as a Gene Mutagenesis Technique

Published:2018-11-08 Issue:24 Volume:19 Page:2542-2544
ISSN:1439-4227
Container-title:ChemBioChem
language:en
Short-container-title:ChemBioChem

Author:

Acevedo‐Rocha Carlos G.¹,Sun Zhoutong²,Reetz Manfred T.³⁴^ORCID

Affiliation:

1. Biosyntia ApS 2100 Copenhagen Denmark

2. Tianjin Institute of Industrial BiotechnologyChinese Academy of Sciences 32 West 7th Avenue, Tianjin Airport Economic Area Tianjin 300308 China

3. Department of ChemistryPhilipps University 35032 Marburg Germany

4. Max-Planck-Institut für Kohlenforschung Kaiser-Wilhelm-Platz 1 45470 Mülheim Germany

Funder

Max-Planck-Society

CAS Pioneer Hundred Talents Program

The Key Projects in the Tianjin Science & Technology Pillar Program

Publisher

Wiley

Subject

Organic Chemistry,Molecular Biology,Molecular Medicine,Biochemistry

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/cbic.201800372

Reference59 articles.

1. A Comparative Reengineering Study of cpADH5 through Iterative and Simultaneous Multisite Saturation Mutagenesis

2. Initial study introducing the term CAST:

3. Expanding the Range of Substrate Acceptance of Enzymes: Combinatorial Active-Site Saturation Test

4. Expanding the Range of Substrate Acceptance of Enzymes: Combinatorial Active-Site Saturation Test

5. Directed Evolution of an Enantioselective Enzyme through Combinatorial Multiple-Cassette Mutagenesis

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