Affiliation:
1. Medical Laboratory Program, Vocational School of Health Services Aksaray University Aksaray Turkey
Abstract
AbstractIn this work, the magnetic cellulose acetate butyrate beads (mCABs) were prepared and then, their surfaces were modified with [(3‐Aminopropyl) triethoxysilane)] (APTES) solution to generate amine groups (−NH2) as a ligand for DNA adsorption in the aqueous solution. After the surface modification, the −NH2 functionalized mCABs (N−mCABs) were characterized with Fourier Transform Infrared Spectroscopy (FTIR), scanning electron microscope (SEM), and energy dispersive X‐ray (EDX) analysis. The optimum pH value of DNA adsorption was observed at pH 4 and at this point, the N−mCABs adsorbed 4.27 mg/g DNA at room temperature (RT). The maximum adsorbed amount of DNA was calculated as 5.24 mg/g and the DNA adsorption was exothermic; furthermore, DNA adsorption was monolayer and the Langmuir adsorption model was more fitted than the Freunclich isotherm model due to the regression coefficient (R2), and the qmax values of the Langmuir model. According to reusability studies, the prepared adsorbent protected its stability and the adsorption capacity after the 5 consecutive adsorption, desorption, and regeneration cycles.