Affiliation:
1. Department of Chemistry Faculty of Science Ondokuz Mayıs University 55139 Samsun Türkiye
2. Research Laboratories Application and Research Center (ALUM) Iğdır University 76000 Iğdır Türkiye
3. Department of Biochemistry Faculty of Science Çankırı Karatekin University 18100 Çankırı Türkiye
4. Science and Technology Application and Research Center Siirt University 56520 Siirt Türkiye
5. Department of Molecular Biology and Genetics Faculty of Arts and Science Bingol University 12000 Bingol Türkiye
6. Department of Pharmaceutical Chemistry Faculty of Pharmacy Ondokuz Mayıs University 55139 Samsun Türkiye
Abstract
AbstractSeveral Nepeta species, used in traditional uses, represent sources of valuable phytochemical composition, as well as in vitro biological and in silico clinical activities. In the present study, the antioxidant and DNA protective properties of methanol: chloroform (1 : 1) extracts of six Nepeta species were evaluated by comparison with standards. In HPLC‐MS/MS analysis, the main components of the extracts were identified as rosmarinic acid, caffeic acid, chlorogenic acid, shikimic acid, and scutellarin. In the GC‐FID analysis, the highest components were palmitate and cis‐11‐eicosenoic acid. N. aristata has the greatest total phenol and flavonoid contents. N. baytopii, N. aristata, N. italica, N. stenantha, and N. nuda exhibited the highest antioxidant activities. The N. nuda, N. aristata, N. italica, and N. trachonitica extracts were shown to have the most potent DNA protection activity. According to the result of the GC‐FID and HPLC‐MS/MS analysis of Nepeta extracts, ADMET studies of the main components of the extracts were performed. It was evaluated that palmitic acid had BBB permeability, but oleic acid was toxic. The in vitro and in silico results confirmed that Nepeta L. extracts are potential natural products.
Funder
Türkiye Bilimsel ve Teknolojik Araştırma Kurumu