Use of the CRISPR‐Cas9 System in Drosophila Cultured Cells to Introduce Fluorescent Tags into Endogenous Genes-Reference-Cited by-同舟云学术

Use of the CRISPR‐Cas9 System in Drosophila Cultured Cells to Introduce Fluorescent Tags into Endogenous Genes

Published:2019-12-23 Issue:1 Volume:130 Page:
ISSN:1934-3639
Container-title:Current Protocols in Molecular Biology
language:en
Short-container-title:Current Protocols in Molecular Biology

Author:

Bosch Justin A.¹,Knight Shannon¹²,Kanca Oguz³⁴,Zirin Jonathan¹²,Yang‐Zhou Donghui¹²,Hu Yanhui¹²,Rodiger Jonathan¹²,Amador Gabriel¹²,Bellen Hugo J.³⁴⁵⁶,Perrimon Norbert¹²⁷,Mohr Stephanie E.¹²

Affiliation:

1. Department of Genetics Harvard Medical School Boston Massachusetts

2. Drosophila RNAi Screening Center Harvard Medical School Boston Massachusetts

3. Department of Molecular and Human Genetics Baylor College of Medicine Houston Texas

4. Jan and Dan Duncan Neurological Research Institute Texas Children's Hospital Houston Texas

5. Department of Neuroscience Baylor College of Medicine Houston Texas

6. Howard Hughes Medical Institute Baylor College of Medicine Houston Texas

7. Howard Hughes Medical Institute Harvard Medical School Boston Massachusetts

Funder

National Science Foundation

National Institute of General Medical Sciences

National Cancer Institute

Damon Runyon Cancer Research Foundation

Howard Hughes Medical Institute

Dana-Farber/Harvard Cancer Center

Publisher

Wiley

Subject

General Medicine

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/cpmb.112

Reference51 articles.

1. Donor DNA Utilization During Gene Targeting with Zinc-Finger Nucleases

2. Gene knock‐ins in Drosophila using homology‐independent insertion of universal donor plasmids;Bosch J. A.;Genetics,2019

3. Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells

4. The transcriptional diversity of 25 Drosophila cell lines

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