Affiliation:
1. Department of Biomedical Engineering College of Engineering Texas A&M University College Station TX 77843 USA
2. Department of Cell Biology and Genetics College of Medicine Texas A&M University Bryan TX 77807‐3260 USA
3. Interdisciplinary Program in Genetics and Genomics Texas A&M University College Station TX 77843 USA
4. Department of Material Science and Engineering College of Engineering Texas A&M University College Station TX 77843 USA
Abstract
AbstractMinerals play a vital role, working synergistically with enzymes and other cofactors to regulate physiological functions including tissue healing and regeneration. The bioactive characteristics of mineral‐based nanomaterials can be harnessed to facilitate in situ tissue regeneration by attracting endogenous progenitor and stem cells and subsequently directing tissue‐specific differentiation. Here, cellular responses of human mesenchymal stem/stromal cells to traditional bioactive mineral‐based nanomaterials, such as hydroxyapatite, whitlockite, silicon‐dioxide, and the emerging synthetic 2D nanosilicates are investigated. Transcriptome sequencing is utilized to probe the cellular response and determine the significantly affected signaling pathways due to exposure to these inorganic nanomaterials. Transcriptome profiles of stem cells treated with nanosilicates reveals a stabilized skeletal progenitor state suggestive of endochondral differentiation. This observation is bolstered by enhanced deposition of matrix mineralization in nanosilicate treated stem cells compared to control or other treatments. Specifically, use of 2D nanosilicates directs osteogenic differentiation of stem cells via activation of bone morphogenetic proteins and hypoxia‐inducible factor 1‐alpha signaling pathway. This study provides insight into impact of nanomaterials on cellular gene expression profile and predicts downstream effects of nanomaterial induction of endochondral differentiation.
Funder
National Institute of Dental and Craniofacial Research
National Institute of Biomedical Imaging and Bioengineering
National Institute of Neurological Disorders and Stroke
Cancer Prevention and Research Institute of Texas
Congressionally Directed Medical Research Programs
National Cancer Institute
Cited by
3 articles.
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