Magnetic‐Assisted Control of Eggs and Embryos via Zona Pellucida‐Linked Nanoparticles

Author:

García‐Vázquez Francisco Alberto12ORCID,Garrappa Gabriela134ORCID,Luongo Chiara1,Hamze Julieta Gabriela23ORCID,Caballero María13,Marco‐Jiménez Francisco5,Vicente Antón José Salvador5,Molina‐Cuberos Gregorio J.6,Jiménez‐Movilla María23

Affiliation:

1. Departamento de Fisiología, Facultad de Veterinaria, Campus de Excelencia Mare Nostrum Universidad de Murcia Murcia 30100 Spain

2. Instituto Murciano de Investigación Biosanitaria (IMIB‐Arrixaca) Murcia 30120 Spain

3. Departamento de Biología Celular e Histología, Facultad de Medicina y Enfermería, Campus de Excelencia Mare Nostrum Universidad de Murcia Murcia 30120 Spain

4. Insitituto Nacional de Tecnología Agropecuaria (INTA) Rafaela Santa Fe 2300 Argentina

5. Instituto de Ciencia y Tecnología Animal Universitat Politècnica de València Valencia 46022 Spain

6. Departamento de Electromagnetismo y Electrónica, Facultad de Química Universidad de Murcia Murcia 30100 Spain

Abstract

AbstractEggs and embryo manipulation is an important biotechnological challenge to enable positioning, entrapment, and selection of reproductive cells to advance into a new era of nature‐like assisted reproductive technologies. Oviductin (OVGP1) is an abundant protein in the oviduct that binds reversibly to the zona pellucida, an extracellular matrix that surrounds eggs and embryos. Here, the study reports a new method coupling OVGP1 to magnetic nanoparticles (NP) forming a complex (NPOv). NPOv specifically surrounds eggs and embryos in a reversible manner. Eggs/embryos bound to NPOv can be moved or retained when subjected to a magnetic force, and interestingly only mature‐competent eggs are attracted. This procedure is compatible with normal development following gametes function, in vitro fertilization, embryo development and resulting in the birth of healthy offspring. The results provide in vitro proof‐of‐concept that eggs and embryos can be precisely guided in the absence of physical contact by the use of magnets.

Funder

Ministerio de Ciencia e Innovación

Ministerio de Ciencia, Innovación y Universidades

Publisher

Wiley

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