A cocktail of protein engineering strategies: Breaking the enzyme bottleneck one by one for high UTP production in vitro
Author:
Affiliation:
1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology Shanghai China
2. Shanghai Collaborative Innovation Center for Biomanufacturing Technology Shanghai China
Funder
National Natural Science Foundation of China
Fundamental Research Funds for the Central Universities
Publisher
Wiley
Subject
Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Link
https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.28061
Reference41 articles.
1. Sustainable synthesis of uridine-5′-monophosphate analogues by immobilized uracil phosphoribosyltransferase from Thermus thermophilus
2. Accurate prediction of protein structures and interactions using a three-track neural network
3. Production of orotic acid by a Klura3 Δ mutant of Kluyveromyces lactis
4. Enzymatic Synthesis of Nucleoside Triphosphates and Deoxynucleoside Triphosphates by Surface-Displayed Kinases
5. UMP kinase from Streptococcus pneumoniae: evidence for co-operative ATP binding and allosteric regulation
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