Comprehensive analysis of cuproptosis‐related gene expression and positive correlations with CD4+ T cell infiltration in head and neck squamous cell carcinoma

Author:

Hu Zhangwei12ORCID,Lei Wenbin12,Wen Weiping123

Affiliation:

1. Department of Otolaryngology, The First Affiliated Hospital Sun Yat‐sen University Guangzhou China

2. Otorhinolaryngology Institute Sun Yat‐sen University Guangzhou China

3. Department of Otolaryngology The Sixth Affiliated Hospital of Sun Yat‐sen University Guangzhou China

Abstract

AbstractCuproptosis is a novel form of copper‐induced programmed cell death. Here, we investigate the role of cuproptosis‐related genes in head and neck squamous cell carcinoma (HNSCC), and the correlations between cuproptosis‐related genes and tumor‐infiltrating immune cells in the microenvironment. Gene expression data were downloaded from TCGA and analyzed using R. Protein–protein interaction analysis was conducted using STRING and GeneMANIA, while survival analyses examined the correlations between genes and overall survival. In addition, analyses of methylation and mutation sites were performed using Illumina HumanMethylation450 data and the cBioPortal data set, respectively. The correlations between gene expression level and infiltrating immune cells and predictive values of PD‐1/PD‐L1 blockade responses were also analyzed. Cuproptosis‐promoting genes were downregulated, while two out of three cuproptosis‐inhibiting genes, GLS and CDKN2A, showed upregulated expression. The CDKN2A expression level was identified as an independent prognostic factor for HNSCC through the multivariate Cox survival analysis. Meanwhile, levels of all cuproptosis‐related genes correlated positively with CD4+ T cell infiltration. Furthermore, expression of the cuproptosis‐promoting gene DLD was negatively correlated with immune score. All cuproptosis‐related genes were expressed aberrantly and correlated positively with CD4+ T cell infiltration in HNSCC. Thus, copper homeostasis and cuproptosis could be targeted therapeutically as a potential means of enhancing the efficacy of immunotherapy.

Funder

National Natural Science Foundation of China

China Postdoctoral Science Foundation

Publisher

Wiley

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3