Preparation of [1′‐13C]citric acid as a probe in a breath test to evaluate tricarboxylic acid cycle flux
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Published:2024-01-03
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Volume:
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ISSN:0362-4803
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Container-title:Journal of Labelled Compounds and Radiopharmaceuticals
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language:en
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Short-container-title:Labelled Comp Radiopharmac
Author:
Mitome Hidemichi1ORCID,
Takenishi Mieko1,
Ono Kumiko1,
Kawagoe Naoyuki2,
Imai Tsunehiko2,
Sasaki Yosuke2,
Urita Yoshihisa2,
Akira Kazuki1
Affiliation:
1. College of Pharmaceutical Sciences Matsuyama University Matsuyama Ehime Japan
2. Department of General Medicine and Emergency Medicine, School of Medicine Toho University Tokyo Japan
Abstract
[1′‐13C]Citric acid (1) was efficiently prepared from dimethyl 1,3‐acetonedicarboxylate in two steps as a probe for a breath test. The synthetic method was selected because of the yield and reproducibility. Compound 1 was orally administrated to rats, and the time course of the increase of 13CO2/12CO2 ratios (Δ13CO2) in their breath was successfully followed, indicating the metabolism of 1. Thus, the 13C‐breath test using 1 is a promising method to evaluate tricarboxylic acid (TCA) cycle flux.
Subject
Organic Chemistry,Spectroscopy,Drug Discovery,Radiology, Nuclear Medicine and imaging,Biochemistry,Analytical Chemistry