Validation of stability‐indicating LC method, degradation study, and impact on antioxidant and antifungal activities of Eugenia uniflora leaves extract
-
Published:2023-08-15
Issue:11
Volume:37
Page:
-
ISSN:0269-3879
-
Container-title:Biomedical Chromatography
-
language:en
-
Short-container-title:Biomedical Chromatography
Author:
dos Santos Ewelyn Cintya Felipe12,
Andrade de Lima Juliana Morais1,
Barbosa Machado Janaína Carla12,
Assunção Ferreira Magda Rhayanny1,
Lira Soares Luiz Alberto12
Affiliation:
1. Laboratory of Pharmacognosy, Department of Pharmaceutical Sciences Federal University of Pernambuco Recife Pernambuco Brazil
2. Post‐Graduate Program in Pharmaceutical Sciences Federal University of Pernambuco Recife Pernambuco Brazil
Abstract
AbstractThe aim of this study is to demonstrate the stability‐indicating capacity of an analytical method for Eugenia uniflora, enhance understanding of the stability of myricitrin, and assess the effect of degradation of spray‐dried extract (SDE) on antioxidant and antifungal activities. Validation of the stability‐indicating method was carried out through a forced degradation study of SDE and standard myricitrin. The antioxidant and antifungal activities of SDE were evaluated both before and after degradation. The quantification method described was found to be both accurate and precise in measuring myricitrin levels in SDE from E. uniflora, with excellent selectivity that confirmed its stability‐indicating capability. The forced degradation study revealed that the marker myricitrin is sensitive to hydrolysis, but generally stable under other stress conditions. By contrast, the standard myricitrin displayed greater susceptibility to degradation under forced degradation conditions. Analysis of the antioxidant activity of SDE before and after degradation showed a negative impact in this activity due to degradation, while no significant effect was observed on antifungal activity. The method described can be a valuable tool in the quality control of E. uniflora, and the findings can assist in determining the optimal conditions and storage of products derived from this species.
Funder
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco
Subject
Clinical Biochemistry,Drug Discovery,Pharmacology,Molecular Biology,General Medicine,Biochemistry,Analytical Chemistry
Reference52 articles.
1. Anvisa. (2015).RDC N° 53 de 4 de Dezembro de 2015. Ministério da Saúde. Brasília: National Health Surveillance Agency.
2. Anvisa. (2017).RDC N° 166 de 24 de Julho de 2017. Ministério da Saúde. Brasília: National Health Surveillance Agency.
3. Anvisa. (2019).RDC N° 318 de 6 de Novembro de 2019. Ministério da Saúde. Brasília: National Health Surveillance Agency.
4. Development of validated stability-indicating assay methods—critical review
5. A stability‐indicating ultra‐fast liquid chromatography method for the assay of the main flavonoids of
Achyrocline satureioides
(Marcela) in porcine skin layers and nanoemulsions