Knockout of IRF3 and IRF7 genes by CRISPR/Cas9 technology enhances porcine virus replication in the swine testicular (ST) cell line

Author:

Sun Xiamei1,Wang Jing1,Mou Chunxiao1,Shi Kaichuang2,Bao Wenbin3,Chen Zhenhai145ORCID

Affiliation:

1. College of Veterinary Medicine Yangzhou University Yangzhou Jiangsu China

2. Guangxi Center for Animal Disease Control and Prevention Nanning Guangxi China

3. Key Laboratory for Animal Genetics Breeding, Reproduction and Molecular Design of Jiangsu Yangzhou Jiangsu China

4. Joint International Research Laboratory of Agriculture and Agri‐Product Safety, the Ministry of Education of China Yangzhou University Yangzhou Jiangsu China

5. Jiangsu Co‐Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou University Yangzhou Jiangsu China

Abstract

AbstractAntiviral vaccines for pig diseases are essential to prevent epidemic outbreaks. However, their production is often hindered by inefficient manufacturing processes that yield lower quantities of the vaccine. To accelerate the progress of various areas of bioproduction, we have considered the necessity of enhancing viral replication efficiency by optimizing ST (swine testicular) cell lines that are commonly utilized in virus manufacturing. CRISPR/Cas9 gene‐editing technology were utilized to create IRF3 or IRF7 knockout cell lines that facilitate high‐titer viral stock production. Compared to the parental cell lines, the ST IRF3/7 KO cell line displayed a compromised antiviral response to a panel of viruses (Porcine epidemic diarrhea virus, Senecavirus A, Parainfluenza virus 5, and Getah virus), as evidenced by decreased expression of interferon and certain antiviral factors. The inhibition of these responses led to heightened viral replication and increased cytopathic effects, ultimately promoting apoptosis. As a result, the development of these cell lines offers a more efficient approach for biopharmaceutical companies to boost their virus production and reduce associated costs.

Funder

National Key Research and Development Program of China

Higher Education Discipline Innovation Project

Priority Academic Program Development of Jiangsu Higher Education Institutions

Publisher

Wiley

Subject

Molecular Medicine,Applied Microbiology and Biotechnology,General Medicine

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