Protein precipitation of diluted samples in SDS-containing buffer with acetone leads to higher protein recovery and reproducibility in comparison with TCA/acetone approach-Reference-Cited by-同舟云学术

Protein precipitation of diluted samples in SDS-containing buffer with acetone leads to higher protein recovery and reproducibility in comparison with TCA/acetone approach

Published:2016-05-23 Issue:13 Volume:16 Page:1847-1851
ISSN:1615-9853
Container-title:PROTEOMICS
language:en
Short-container-title:Proteomics

Author:

Santa Cátia¹²,Anjo Sandra I.¹³,Manadas Bruno¹

Affiliation:

1. Center for Neuroscience and Cell Biology; University of Coimbra; Coimbra Portugal

2. Institute for Interdisciplinary Research; University of Coimbra; Coimbra Portugal

3. Faculty of Sciences and Technology; University of Coimbra; Coimbra Portugal

Funder

Fundação para a Ciência e Tecnologia (FCT)

QREN

FEDER - Fundo Europeu de Desenvolvimento Regional

RNEM

Publisher

Wiley

Subject

Molecular Biology,Biochemistry

Reference15 articles.

1. Maximizing recovery of water-soluble proteins through acetone precipitation;Crowell;Anal. Chim. Acta,2013

2. The use of acetone precipitation in the isolation of ribosomal proteins;Barritault;Eur. J. Biochem. / FEBS,1976

3. Top-down and bottom-up proteomics of SDS-containing solutions following mass-based separation;Botelho;J. Proteome Res.,2010

4. Extracellular vesicles are rapidly purified from human plasma by Protein Organic Solvent Precipitation (PROSPR);Gallart-Palau;Sci. Rep.,2015

5. A new use for existing technology-continuous precipitation for purification of recombination proteins;Warikoo;Biotechnol. J.,2015

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