Affiliation:
1. School of Chemical and Biomolecular Engineering Georgia Institute of Technology Atlanta GA 30332 USA
2. Parker H. Petit Institute for Bioengineering and Biosciences Georgia Institute of Technology Atlanta GA 30332 USA
3. George W. Woodruff School of Mechanical Engineering Georgia Institute of Technology Atlanta GA 30332 USA
Abstract
AbstractHuman intestinal organoids (HIOs) derived from pluripotent stem cells or adult stem cell biopsies represent a powerful platform to study human development, drug testing, and disease modeling in vitro, and serve as a cell source for tissue regeneration and therapeutic advances in vivo. Synthetic hydrogels can be engineered to serve as analogs of the extracellular matrix to support HIO growth and differentiation. These hydrogels allow for tuning the mechanical and biochemical properties of the matrix, offering an advantage over biologically derived hydrogels such as Matrigel. Human intestinal organoids have been used for repopulating transplantable intestinal grafts and for in vivo delivery to an injured intestinal site. The use of synthetic hydrogels for in vitro culture and for in vivo delivery is expected to significantly increase the relevance of human intestinal organoids for drug screening, disease modeling, and therapeutic applications.
Funder
National Science Foundation
National Institute of Diabetes and Digestive and Kidney Diseases
Subject
Mechanical Engineering,Mechanics of Materials,General Materials Science