Affiliation:
1. Researcher in Biology and Animal Reproduction Urmia Iran
2. Department of Theriogenology, Faculty of Veterinary Medicine Urmia University Urmia Iran
3. Republic of Turkey Ministry of Agriculture and Foresty International Center for Livestock Research and Training Ankara Turkey
4. Department of Reproductive Biotechnologies and Cryobiology of Gametes Institute of Biology and Immunology of Reproduction ‘Acad. Kiril Bratanov’ at Bulgarian Academy of Sciences Sofia Bulgaria
5. Faculty of Veterinary Medicine Department of Biochemistry Afyon Kocatepe University Afyonkarahisar Turkey
6. ACR Bio Food and Biochemistry Research and Development Afyonkarahisar Turkey
7. Faculty of Veterinary Medicine Kyrgyz‐Turkish Manas University Bishkek Kyrgyzstan
8. Faculty of Agriculture, Department of Animal Science College of Agriculture and Natural Resources University of Tehran Karaj Iran
9. Faculty of Veterinary Medicine Department of Reproduction and Artificial Insemination Clinical Sciences, Istanbul University‐Cerrahpasa Avcilar‐Istanbul Turkey
10. Faculty of Veterinary Medicine Department of Obstetrics and Gynecology University of Selcuk Konya Turkey
Abstract
AbstractBackgroundThis study investigates the effects of cryopreservation and supplementation of Azeri water buffalo's semen with proline (Lp) and fulvic acid (FA).ObjectivesTherefore, this study aimed to assess motility parameters, sperm viability, oxidative stress parameters, and DNA damage to detect the optimum concentrations of Lp and FA for buffalo semen cryopreservation.MethodsThirty semen samples of three buffalo bulls were diluted in Tris‐egg yolk extender and divided into 12 equal groups including control (C), Lp‐10, Lp‐20, Lp‐40, Lp‐60, Lp‐80 (containing 10, 20, 40, 60, 80 mM L‐proline, respectively), FA‐0.2, FA‐0.5, FA‐0.8, FA‐1.1, FA‐1.4 and FA‐1.7 (containing 0.2%, 0.5%, 0.8%, 1.1%, 1.4% and 1.7% fulvic acid, respectively).ResultsThe velocity parameters, TM and PM were improved by FA‐1.7, FA‐1.4, Lp‐40 and Lp‐60 groups compared to the C group but no significant difference was found regarding the amplitude of lateral head displacement and straightness compared to the control groups. The percentage of sperm viability and PMF were increased by FA‐1.7, FA‐1.4, FA‐1.1, Lp‐40 and Lp‐60 groups compared to C group, while in terms of sperm DNA damage FA‐1.7, FA‐1.4, FA‐1.1, Lp‐10, Lp‐20, Lp‐40 and Lp‐60 groups showed better results compared to C group. The results also showed that FA‐1.7, FA‐1.4, FA‐1.1, Lp‐20, Lp‐40 and Lp‐60 groups could improve TAC, SOD, GSH and decrease MDA levels. Also, FA‐1.7, FA‐1.4, Lp‐20 and Lp‐40 groups could improve GPx levels but just FA‐1.7, and Lp‐40 groups could improve CAT levels compared to C group.ConclusionsThus, it can be concluded that L‐proline and fulvic acid supplementations can improve the quality parameters of post‐thawed buffalo bull semen.