A simple method to immunopurify erythropoiesis stimulating agents from urine, aiming to optimize erythropoietin screening by SAR‐PAGE
Author:
Affiliation:
1. Doping Control Laboratory, Department of Clinical PharmacologyKarolinska University Hospital Stockholm Sweden
2. Department of Laboratory MedicineKarolinska Institutet Stockholm Sweden
Publisher
Wiley
Subject
Spectroscopy,Pharmaceutical Science,Environmental Chemistry,Analytical Chemistry
Link
https://onlinelibrary.wiley.com/doi/pdf/10.1002/dta.2730
Reference17 articles.
1. Detection of isoelectric profiles of erythropoietin in urine: differentiation of natural and administered recombinant hormones
2. SDS-PAGE of recombinant and endogenous erythropoietins: benefits and limitations of the method for application in doping control
3. SARCOSYL‐PAGE: optimized protocols for the separation and immunological detection of PEGylated proteins;Reichel C;Methods Mol Biol,1855
4. Optimizing SAR-PAGE
5. World Anti‐Doping Agency.WADA Technical Document: Harmonization of analysis and reporting of erythropoiesis stimulating agents (ESAs) by electrophoretic techniques.https://www.wada-ama.org/sites/default/files/resources/files/WADA-TD2014EPO-v1-Harmonization-of-Analysis-and-Reporting-of-ESAs-by-Electrophoretic-Techniques-EN.pdf2014[cited 29 July 2019].
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