Affiliation:
1. School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine Tianjin China
2. Anhui Province Key Laboratory of Resource Insect Biology and Innovative Utilization, School of Life Sciences Anhui Agricultural University Hefei China
Abstract
AbstractUbiquitin‐fold modifier 1 (UFM1) is attached to protein substrates through the sequential activity of an E1 (UBA5)‐E2 (UFC1)‐E3 (UFL1) cascade. UFL1 is the E3 ligase for UFMylation in vertebrates. However, there have been no studies on UFL1 in silkworm to date. In this study, we identified a UFL1 ortholog in Bombyx mori genome. Spatio‐temporal expression profiles showed that BmUFL1 expression was high in the midgut, epidermis, and testis and in the pupa–adult stage. BmUFL1 knockdown inhibited B. mori nucleopolyhedrovirus (BmNPV) proliferation, while BmUFL1 overexpression promoted BmNPV proliferation. Mechanically, protein kinase RNA‐like endoplasmic reticulum kinase (PERK) signaling and cell apoptosis are involved in BmUFL1‐regulated BmNPV proliferation. Overall, these results suggest that BmUFL1 facilitates BmNPV proliferation in silkworm.