LC‐1000 flow cytometry system complements intraoperative peritoneal cytology for pancreatic and biliary tract cancer

Author:

Asakura Riki1ORCID,Ajiki Tetsuo12ORCID,Abe Shigeki3,Yanagimoto Hiroaki1ORCID,Tsugawa Daisuke1,Komatsu Shohei1ORCID,Goto Tadahiro1,Asari Sadaki12,Toyama Hirochika1,Fukumoto Takumi1

Affiliation:

1. Division of Hepato‐Biliary‐Pancreatic Surgery, Department of Surgery Kobe University Graduate School of Medicine Kobe Japan

2. Division of Hepato‐Biliary‐Pancreatic Surgery International Clinical Cancer Research Center, Kobe University Hospital Kobe Japan

3. Sysmex Corporation Kobe Japan

Abstract

AbstractBackgroundThe exfoliative cell analyzer, LC‐1000, is medical device that utilizes the principles of flow cytometry, and might provide digital diagnostic information for cytology using a different approach from conventional cytomorphology. In this study, wae examined the usefulness of the LC‐1000 as a diagnostic support system for intraoperative peritoneal lavage cytology and its prognostic impact for pancreatic (PC) and biliary tract cancer (BTC).MethodsPatients with PC and BTC who underwent surgical treatment were included. First, we identified useful indicators of LC‐1000 and established cutoff values to discriminate positive cytology. Next, we verified the validity of these cutoff values.ResultsIn the test set (n = 48), of the LC‐1000 indicators examined, only MR‐CPIx was significantly different between the negative and positive cytology groups, yielding a cutoff value of 0.86. In the validation set (n = 52), the sensitivity, specificity, positive and negative predictive value of the LC‐1000 for cytology results was 1.0, 0.49, 0.11 and 1.0, respectively. In patients who had undergone radical resection, recurrence‐free survival rate was significantly higher in the LC‐1000 negative group than in the positive group in PC, but not in BTC.ConclusionThe LC‐1000 was useful as digital support system for peritoneal cytology, and it might have potential as a prognostic factor for PC.

Publisher

Wiley

Subject

Hepatology,Surgery

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