Preparation and characterization of a novel dual-retention mechanism mixed-mode stationary phase with PEG 400 and succinic anhydride as ligand for protein separation in WCX and HIC modes

Author:

Song Chao1,Wang Jianshan1,Zhao Kailou1,Bai Quan1

Affiliation:

1. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Institute of Modern Separation Science, Key Lab of Modern Separation Science in Shaanxi Province; Northwest University; Xi'an 710069 China

Publisher

Wiley

Subject

Clinical Biochemistry,Drug Discovery,Pharmacology,Molecular Biology,General Medicine,Biochemistry,Analytical Chemistry

Reference42 articles.

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3. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding;Bradford;Analytical Biochemistry,1976

4. Use of oxiranes in the preparation of bonded phase supports;Chang;Journal of Chromatography,1976

5. Recovery of recombinant glucosidase by expanded bed adsorption from Pichia pastoris high-cell-density culture broth;Charoenrat;Journal of Biotechnology,2006

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