Affiliation:
1. State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education Guizhou University Guiyang China
2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro‐products, Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences Hangzhou China
3. Jinhua Academy of Agricultural Sciences Jinhua China
4. Technical Centre for Animal, Plant, and Food Inspection and Quarantine Shanghai Customs Shanghai China
Abstract
AbstractThe antioxidant proteins, peroxiredoxins (Prxs), function to protect insects from reactive oxygen species‐induced toxicity. In this study, two Prx genes, CsPrx5, and CsPrx6, were cloned and characterized from the paddy field pest, Chilo suppressalis, containing open reading frames of 570 and 672 bp encoding 189 and 223 amino acid polypeptides, respectively. Then, we investigated the influence of various stresses on their expression levels using quantitative real‐time PCR (qRT‐PCR). The results showed expression of CsPrx5 and CsPrx6 in all developmental stages, with eggs having the highest level. CsPrx5 and CsPrx6 showed higher expression in the epidermis and fat body, and CsPrx6 also showed higher expression in midgut, fat body, and epidermis. Increasing concentrations of insecticides (chlorantraniliprole and spinetoram) and hydrogen peroxide (H2O2) increased the expression levels of CsPrx5 and CsPrx6. In addition, the expression levels of CsPrx5 and CsPrx6 were almost markedly upregulated in larvae under temperature stress or fed by vetiver. Thus, CsPrx5 and CsPrx6 upregulation might increase the C. suppressalis defense response by reducing the impact of environmental stress, providing a better understanding of the relationship between environmental stresses and insect defense systems.
Subject
Insect Science,General Medicine,Biochemistry,Physiology
Cited by
1 articles.
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