Affiliation:
1. College of Biosystems Engineering and Food Science, National‐Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro‐Food Processing, Zhejiang Engineering Laboratory of Food Technology and Equipment, Fuli Institute of Food Science Zhejiang University Hangzhou 310058 China
2. Innovation Center of Yangtze River Delta Zhejiang University Jiashan 314100 China
3. Department of Food Science and Technology Zhejiang University of Technology Hangzhou 310014 China
4. College of Animal Sciences Zhejiang University Hangzhou 310014 China
5. Department of Agricultural, Food and Nutritional Science, 4–10 Ag/For Building University of Alberta Edmonton Alberta T6G 2P5 Canada
Abstract
ScopeTwo peptides VY (Val‐Tyr) and SFLLR (Ser‐Phe‐Leu‐Leu‐Arg) are recently identified from soy‐fermented douchi with hypoglycemic activity in cells. The study aims to understand their potential effects on glucose metabolism and insulin sensitivity as well as their mechanisms of action in a high‐fat diet (HFD) induced insulin resistant model.Methods and resultsC57BL/6 mice are fed HFD for 8 weeks, followed by peptide supplementation (doses: 10 and 50 mg kg−1 body weight) for 8 weeks. Peptides supplementation, especially SFLLR, reduces body weight gain, insulin resistance, hyperglycemia, inflammation, liver injury, and lipid accumulation. In both muscle and liver, both peptides activate glycogen synthase (GS), the key enzyme for glycogen synthesis, and also inhibit phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6‐phosphatase (G6PC), two rate‐limiting enzymes for gluconeogenesis, via insulin and AMPK (5'‐adenosine monophosphate‐activated protein kinase) signaling pathways. Furthermore, VY and SFLLR supplementation reverse HFD‐induced gut dysbacteriosis by decreasing the abundance of Enterococcus, Oscillibacter, and Deferribacter, and also increase the abundances of Alistipes, Lactobacillus, Faecalibaculum, Akkermansia, and Bifidobacterium (usually beneficial in the intestine).ConclusionThe study reveals the potential applications of peptides VY and SFLLR as a diet‐based strategy for the prevention of type 2 diabetes.
Subject
Food Science,Biotechnology
Cited by
6 articles.
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