Yogurt Prevents Colorectal Tumorigenesis in ApcMin/+ Mice

Author:

Li Xiaojing12ORCID,Qian Jin12,Liu Qinglong12,Guo Min12,Zhang Hao12,Li Haitao12ORCID,Chen Wei12

Affiliation:

1. State Key Laboratory of Food Science and Resources Jiangnan University Wuxi 214122 China

2. School of Food Science and Technology Jiangnan University Wuxi 214122 China

Abstract

ScopeYogurt consumption is related to a decreased risk of colorectal cancer (CRC), but whether such association is causal remains unclear. Patients with familial adenomatous polyposis (FAP) are at increased risk of CRC development. Here, the study investigates the efficacy of yogurt for intestinal polyposis chemoprevention in ApcMin/+ mice, a preclinical model for human FAP.Methods and resultsA 10‐week yogurt supplementation (15 g kg−1) in ApcMin/+ mice significantly reduces the intestinal polyp number (6.50 ± 0.97 versus 1.80 ± 0.49; p < 0.001) compared to controls. 16S rRNA gene‐based microbiota analysis suggests that yogurt supplementation may greatly modulate the gut microbiome composition, especially in the relative abundance of Lactobacillus and Bifidobacterium. Importantly, the fecal concentration of d‐lactate (d‐Lac, 0.39 ± 0.04 µmol g−1 versus 8.14 ± 0.62 µmol g−1; p < 0.001) is boosted by yogurt, while oral administration with d‐Lac (125 or 250 mg kg−1) reduces the polyp number by 71.43% or 77.14% (p < 0.001), respectively. The study also observes that d‐Lac does not affect cell viability and anchorage‐independence in CRC cells, but it greatly suppresses epidermal growth factor (EGF) or 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA)‐induced cell transformation in preneoplastic cells. Mechanistically, it demonstrates that d‐Lac may attenuate epithelial cell transformation by targeting PI3K/AKT/β‐catenin axis.ConclusionYogurt protects against intestinal polyposis in ApcMin/+ mice, and d‐Lac may partially account for the chemopreventive effects above.

Funder

National Natural Science Foundation of China

Publisher

Wiley

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3