Chicken Muscle‐Derived ACE2‐Upregulating Peptide VVHPKESF Reduces Blood Pressure Associated with the ACE2/Ang (1‐7)/MasR Axis in Spontaneously Hypertensive Rats

Author:

Fan Hongbing123ORCID,Shang Nan1ORCID,Davidge Sandra T.2456,Wu Jianping12

Affiliation:

1. Department of Agricultural Food and Nutritional Science University of Alberta 4‐10 Ag/For Building Edmonton Alberta T6G 2P5 Canada

2. Cardiovascular Research Centre University of Alberta Edmonton Alberta T6G 2R7 Canada

3. Department of Animal and Food Sciences University of Kentucky Lexington KY 40546 USA

4. Department of Obstetrics and Gynecology University of Alberta Edmonton Alberta T6G 2R7 Canada

5. Department of Physiology University of Alberta Edmonton Alberta T6G 2R7 Canada

6. Women and Children's Health Research Institute University of Alberta Edmonton Alberta T6G 2R7 Canada

Abstract

ScopeThis study aims to investigate the antihypertensive effect of four chicken muscle‐derived angiotensin (Ang)‐converting enzymes (ACE)‐regulating peptides: Val‐Arg‐Pro (VRP, ACE inhibition), Leu‐Lys‐Tyr and Val‐Arg‐Tyr (LKY and VRY, ACE inhibition and ACE2 upregulation), and Val‐Val‐His‐Pro‐Lys‐Glu‐Ser‐Phe (VVHPKESF [V‐F], ACE2 upregulation) in spontaneously hypertensive rats.Methods and resultsRats (12–14 weeks old) are grouped: 1) untreated, 2) VRP, 3) LKY, 4) VRY, and 5) V‐F. Blood pressure (BP) is monitored using implantable telemetry technology. Over 18‐day oral administration of 15 mg kg−1 body weight (BW) per day, only peptide V‐F significantly (p < 0.05) reduces BP, decreases circulating Ang II, and increases ACE2 and Ang (1‐7) levels, and enhances aortic expressions of ACE2 and Mas receptor (MasR). Peptide V‐F also attenuates vascular inflammation (TNFα, MCP‐1, IL‐1α, IL‐15, and cyclooxygenase 2 [COX2]) and vascular oxidative stress (nitrotyrosine). The gastrointestinal (GI)‐degraded fragment of peptide V‐F, Val‐Val‐His‐Pro‐Lys (VVHPK), is also an ACE2‐upregulating peptide. Peptides VRP, LKY, and VRY do not reduce BP, possibly due to low bioavailability or other unknown reasons.ConclusionsPeptide V‐F is the first ACE2‐upregulating peptide, purified and fractionated from food proteins based on in vitro ACE2 upregulation, that reduces BP associated with the activation of ACE2/Ang (1‐7)/MasR axis; the N‐terminal moiety VVHPK may be responsible for the antihypertensive effect of V‐F.

Funder

Natural Sciences and Engineering Research Council of Canada

Egg Farmers of Canada

Alberta Innovates - Technology Futures

Killam Trusts

American Oil Chemists' Society

Publisher

Wiley

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3