Burkholderia ambifaria H8 as an effective biocontrol strain against maize stalk rot via producing volatile dimethyl disulfide

Author:

Chen Xinyu1,Liu Jingrong2,Chen Amanda Juan3,Wang Lin3,Jiang Xianzhi3,Gong Andong2,Liu Wende1,Wu Hanxiang1ORCID

Affiliation:

1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Integrated Pest Management in Crops Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Science Beijing China

2. College of Life Science, Xinyang Normal University Xinyang China

3. Microbiome Research Center, Moon (Beijing) Biotech Ltd. Beijing P.R. China

Abstract

AbstractBackgroundMaize stalk rot (MSR) caused by Fusarium graminearum is the primary factor contributing to the reduction in maize yield and quality. However, this soil‐borne disease presents a significant challenge for sustainable control through field management and chemical agents. The screening of novel biocontrol agents can aid in developing innovative and successful strategies for MSR control.ResultsA total of 407 strains of bacteria were isolated from the rhizosphere soil of a resistant maize inbred line. One strain exhibited significant antagonistic activity in plate and pot experiments, and was identified as Burkholderia ambifaria H8. The strain could significantly inhibit the mycelial growth and spore germination of F. graminearum, induce resistance to stalk rot, and promote plant growth. The volatile compounds produced by strain H8 and its secondary metabolites in the sterile fermentation broth exhibited antagonistic activity. The primary volatile compound produced by strain H8 was identified as dimethyl disulfide (DMDS) using gas chromatography tandem mass spectrometry. Through in vitro antagonistic activity assays and microscopic observation, it was confirmed that DMDS was capable of inhibiting mycelial growth and disrupting the mycelial structure of F. graminearum, suggesting it may be the major active compound for strain H8. The transcriptome data of F. graminearum further indicated that strain H8 and its volatile compounds could alter pathogenic fungi metabolism, influence the related metabolic pathways, and potentially induce cell apoptosis within F. graminearum.ConclusionOur results showed that B. ambifaria H8 was capable of producing the volatile substance dimethyl disulfide, which influenced the synthesis and permeability of cell membranes in pathogens. Thus, B. ambifaria H8 was found to be a promising biological control agent against MSR. © 2024 Society of Chemical Industry.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

Agricultural Science and Technology Innovation Program

Publisher

Wiley

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