Analytical method for urinary homovanillic acid and 5‐hydroxyindoleacetic acid levels using HPLC with electrochemical detection applied to evaluate children environmentally exposed to manganese

Author:

Cardoso Mariana Silva1,Rocha Andrea Rebouças2,Souza‐Júnior José Antônio3,Menezes‐Filho José Antonio123ORCID

Affiliation:

1. Graduate Program in Pharmacy, College of Pharmacy Federal University of Bahia Salvador Brazil

2. Graduate Program in Food Science, College of Pharmacy Federal University of Bahia Salvador Brazil

3. Laboratory of Toxicology, College of Pharmacy Federal University of Bahia Salvador Brazil

Abstract

AbstractHomovanillic acid (HVA) and 5‐hydroxyindoleacetic acid (5‐HIAA) are the urinary metabolites of dopamine (DA) and serotonin (5‐HA), respectively. We aimed to develop an extraction method for the determination of HVA and 5‐HIAA, using strong anionic exchange cartridges combined with HPLC with electrochemical detection, and apply it to measure the levels of HVA and 5‐HIAA in children living near a ferro‐manganese alloy plant in Simões Filho, Brazil. The validated method showed good selectivity, sensitivity, precision, and accuracy. The limits of detection (LOD) were 4 and 8 μmol/L for 5‐HIAA and HVA, respectively, in urine. Recoveries ranged from 85.8 to 94%. The coefficients of determination (R2) of the calibration curves were greater than 0.99. Spot urine samples of 30 exposed children and 20 nonexposed ones were processed accordingly. The metabolite levels in exposed and reference children were within the physiological ranges. The medians (range) for 5‐HIAA and HVA of the exposed ones were 36.4 μmol/L (18.4–58.0) and 32.9 μmol/L (<LOD—91.9), respectively. There was no significant difference between the values presented by children in the reference group: 25.7 μmol/L (19.9–81.4) and 35.2 μmol/L (<LOD—67.6) for 5‐HIAA and HVA, respectively. These results suggest that the quantification of the urinary metabolites possibly does not reflect the interference of manganese in the metabolism of DA and 5‐HA in the central nervous system.

Funder

Fundação de Amparo à Pesquisa do Estado da Bahia

Publisher

Wiley

Subject

Clinical Biochemistry,Drug Discovery,Pharmacology,Molecular Biology,General Medicine,Biochemistry,Analytical Chemistry

Reference42 articles.

1. ABEP (Associação Brasileira de Estudos Populacionais). (2018).Critério de Classificação Econômica Brasil.

2. Neuromythology of Manganism

3. ANVISA (Agência Nacional de Vigilância Sanitária). (2012).Resolução da Diretoria Colegiada no 27/2012.

4. ANVISA (Agência Nacional de Vigilância Sanitária). (2017).Resolução da Diretoria Colegiada n° 166/2017.

5. Bonfigli A. R. Coppa G. Testa R. Testa I. &De Sio G.(1997).Determination of vanillylmandelic 5‐hydroxyindoleacetic and homovanillic acid in urine by isocratic liquid chromatography.

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