Affiliation:
1. State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock Inner Mongolia Key Laboratory for Molecular Regulation of the Cell Institute of Biomedical Sciences School of Life Sciences Inner Mongolia University Hohhot 010020 P .R. China
2. Shandong Provincial Key Laboratory of Animal Resistance Biology Key Laboratory of Food Nutrition and Safety of Shandong Normal University College of Life Science Shandong Normal University Jinan 250014 P. R. China
Abstract
AbstractThe limitations of protein‐based hydrogels, including their insufficient mechanical properties and restricted biological functions, arise from the highly specific functions of proteins as natural building blocks. A potential solution to overcome these shortcomings is the development of protein–protein hydrogels, which integrate structural and functional proteins. In this study, a protein–protein hydrogel formed by crosslinking bovine serum albumin (BSA) and a genetically engineered intrinsically disordered collagen‐like protein (CLP) through Ag─S bonding is introduced. The approach involves thiolating lysine residues of BSA and crosslinking CLP with Ag+ ions, utilizing thiolation of BSA and the free‐cysteines of CLP. The resulting protein–protein hydrogels exhibit exceptional properties, including notable plasticity, inherent self‐healing capabilities, and gel–sol transition in response to redox conditions. In comparison to standalone BSA hydrogels, these protein–protein hydrogels demonstrate enhanced cellular viability, and improved cellular migration. In vivo experiments provide conclusive evidence of accelerated wound healing, observed not only in murine models with streptozotocin (Step)‐induced diabetes but also in zebrafish models subjected to UV‐burn injuries. Detailed mechanistic insights, combined with assessments of proinflammatory cytokines and the expression of epidermal differentiation‐related proteins, robustly validate the protein–protein hydrogel's effectiveness in promoting wound repair.
Funder
Natural Science Foundation of Inner Mongolia Autonomous Region
National Natural Science Foundation of China
Cited by
4 articles.
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