Affiliation:
1. Interdisciplinary Program in Bioengineering Seoul National University Seoul 08826 Republic of Korea
2. School of Chemical and Biological Engineering Seoul National University Seoul 08826 Republic of Korea
3. Department of Social Welfare Seoul National University Seoul 08826 Republic of Korea
4. Cellular Reprogramming and Embryo Biotechnology Laboratory Dental Research Institute Seoul National University School of Dentistry Seoul 08826 Republic of Korea
5. BioMax/N‐Bio Institute Seoul National University Seoul 08826 Republic of Korea
6. Institute of Engineering Research Seoul National University Seoul 08826 Republic of Korea
Abstract
AbstractAlthough the polyphenols have been studied to alleviate inflammation, there are still challenges to delivering the polyphenols with stabilized formulation due to their low water solubility and susceptibility to oxidation. Herein, the transdermal delivery system of polyphenol mixture (PM), including quercetin (Q), phloretin (P), and ellagic acid (E), is developed using double emulsion for applying to atopic dermatitis (AD). Through the in vitro anti‐degranulation assay, the optimal molar ratio of each polyphenol (Q:P:E = 5:1:1) is obtained, and the PM shows at most a 43.6% reduction of degranulation of immune cells, which is the primary factor of AD. Moreover, the water‐in‐oil‐in‐water double emulsion (W/O/W) enhances the PM's stability and has a higher anti‐degranulation effect than the oil‐in‐water emulsion (O/W). In the in vivo 1‐chloro‐2,4‐dinitrobenzene (DNCB)‐induced mice AD model, PM reduces more AD symptoms than every single polyphenol. The PM‐encapsulated W/O/W (PM_W/O/W) shows the most effectiveness in AD by decreasing dermatitis score, i.e., skin/ear thickness, mast cells, and serum IgE level. Finally, this suggests that the findings on the optimal ratio of PM and double emulsion‐based delivery would be beneficial in treating AD and can be applied to other allergic diseases.
Subject
Pharmaceutical Science,Biomedical Engineering,Biomaterials
Cited by
1 articles.
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