Affiliation:
1. School of Public Health Guangxi Medical University Nanning China
2. Center for Genomic and Personalized Medicine Guangxi Key Laboratory for Genomic and Personalized Medicine Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine Guangxi Medical University Nanning China
3. Department of Urology Institute of Urology and Nephrology The First Affiliated Hospital of Guangxi Medical University Nanning China
Abstract
AbstractBackgroundThe plasticity of endothelial cells (ECs) is crucial for tissue response to injury. Myocardial infarction can profoundly affect EC function, leading to a shift toward mesenchymal differentiation.MethodsWe utilized human single‐nucleus RNA sequencing data to investigate the dynamic changes and cellular interactions between normal and post‐infarction ECs.ResultsWe identified two distinct subpopulations of ECs: A transient subpopulation characterized by short‐term mesenchymal gene expression and a long‐term subpopulation characterized by myocardial gene expression. Trajectory analysis revealed the differentiation pathways and potential roles over time and space. Furthermore, we uncovered the proliferation, differentiation, hypoxic, and inflammatory responses of ECs to injury.ConclusionsOur study provides a comprehensive and detailed characterization of endothelial cell states, highlighting the role of activated endothelial cell subpopulations in promoting inflammation and tissue repair after infarction.