A Potentiometric Dual‐Channel Microsensor Reveals that Fluctuation of H2S is Less pH‐Dependent During Spreading Depolarization in the Rat Brain-Reference-Cited by-同舟云学术

A Potentiometric Dual‐Channel Microsensor Reveals that Fluctuation of H2S is Less pH‐Dependent During Spreading Depolarization in the Rat Brain

Published:2024-01-25 Issue: Volume: Page:
ISSN:1433-7851
Container-title:Angewandte Chemie International Edition
language:en
Short-container-title:Angew Chem Int Ed

Author:

Liu Rantong¹,Zhang Shuai¹,Zeng Hui¹,Gao Nan¹,Yin Yongyue²,Zhang Meining³,Mao Lanqun⁴

Affiliation:

1. Renmin University of China Department of Chemistry CHINA

2. Renmin University of China Library Department of Chemistry CHINA

3. Renmin University of China Department of Chemistry zhongguancun street 59th 100872 Beijing CHINA

4. Beijing Normal University College of Chemistry CHINA

Abstract

Spreading depolarization (SD) is one of the most common neuropathologic phenomena in the nervous system, relating to numerous diseases. However, real‐time monitoring the rapid chemical changes during SD to probe the molecular mechanism remains a great challenge. We develop a potentiometric dual‐channel microsensor for simultaneous monitoring of H2S and pH featuring excellent selectivity and spatiotemporal resolution. Using this microsensor we firstobserve real time changes of H2S and pH in the rat brain induced by SD. This changes of H2S are completely suppressed when the rat pre‐treats with aminooxyacetic acid (AOAA), a blocker to inhibit the H2S‐producing enzyme, indicating H2S fluctuation might be related to enzyme‐dependent pathway during SD and less pH‐dependent. This study provides a new perspective for studying the function of H2S and the molecular basis of SD‐associated diseases.

Publisher

Wiley

Subject

General Chemistry,Catalysis

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.202318973