An Optochemical Oxygen Scavenger Enabling Spatiotemporal Control of Hypoxia

Author:

Ieda Naoya1ORCID,Sawada Masato23ORCID,Oguchi Runa1,Itoh Masato1,Hirakata Seina4,Saitoh Daisuke1,Nakao Akito5ORCID,Kawaguchi Mitsuyasu1ORCID,Sawamoto Kazunobu23ORCID,Yoshihara Toshitada4ORCID,Mori Yasuo5,Nakagawa Hidehiko1ORCID

Affiliation:

1. Graduate School of Pharmaceutical Sciences Nagoya City University 3-1, Tanabe-dori, Mizuho-ku, Nagoya-shi Aichi 467-8603 Japan

2. Graduate School of Medical Sciences Nagoya City University 1, Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya-shi Aichi 467-8601 Japan

3. Division of Neural Development & Regeneration National Institute for Physiological Sciences 38, Nishigonaka, Myodaiji-cho, Okazaki-shi Aichi 444-8585 Japan

4. Graduate School of Science and Technology Gunma University 1-5-1, Tenjin-cho, Kiryu-shi Gunma 376-8515 Japan

5. Graduate School of Engineering Kyoto University Katsura, Katsura, Nishikyo-ku Kyoto 615-8510 Japan

Abstract

AbstractWe present an optochemical O2 scavenging system that enables precise spatiotemporal control of the level of hypoxia in living cells simply by adjusting the light intensity in the illuminated region. The system employs rhodamine containing a selenium or tellurium atom as an optochemical oxygen scavenger that rapidly consumes O2 by photochemical reaction with glutathione as a coreductant upon visible light irradiation (560–590 nm) and has a rapid response time, within a few minutes. The glutathione‐consuming quantum yields of the system were calculated as about 5 %. The spatiotemporal O2 consuming in cultured cells was visualized with a hypoxia‐responsive fluorescence probe, MAR. Phosphorescence lifetime imaging was applied to confirmed that different light intensities could generate different levels of hypoxia. To illustrate the potential utility of this system for hypoxia research, we show that it can spatiotemporally control calcium ion (Ca2+) influx into HEK293T cells expressing the hypoxia‐responsive Ca2+ channel TRPA1.

Funder

Japan Society for the Promotion of Science

ACT-X

Nagoya City University

Japan Agency for Medical Research and Development

Publisher

Wiley

Subject

General Chemistry,Catalysis

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