N-terminal processing of affinity-tagged recombinant proteins purified by IMAC procedures

Author:

Mooney Jane T.1,Fredericks Dale P.1,Christensen Thorkild2,Bruun Schiødt Christine2,Hearn Milton T. W.1

Affiliation:

1. Centre for Green Chemistry, School of Chemistry; Monash University; Clayton Victoria 3800 Australia

2. Novo Nordisk A/S; Novo Nordisk Park DK-2760 Måløv Denmark

Funder

Australian Research Council

Publisher

Wiley

Subject

Molecular Biology,Structural Biology

Reference77 articles.

1. Equilibrium binding of peptides to immobilized Cu2+;Andersson;Int. J. Bio-Chromotogr.,1996

2. Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins;Arnau;Prot. Exp. Purif.,2006

3. Metal-Affinity Separations: a New Dimension in Protein Processing;Arnold;Bio/Technology,1991

4. Protease-immobilized microreactor for rapid and site-specific affinity tag cleavage;Asanomi;Pept. Sci.,2011

5. Immobilized-metal affinity chromatography (IMAC);Block;Meths Enzymol.,2009

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