Solution NMR structure of cementum protein 1 derived peptide (CEMP1‐p1) and its role in the mineralization process

Author:

Campos Mikado Nidome1,Giraldo Estefanía López2,del Rio Portilla Federico3,Fernández‐Velasco Daniel Alejandro4,Arzate Higinio1ORCID,Romo‐Arévalo Enrique1ORCID

Affiliation:

1. Laboratorio de Biología Periodontal, Facultad de Odontología Universidad Nacional Autónoma de México Mexico City Mexico

2. Chemistry Department University of Colorado Denver Denver CO USA

3. Instituto de Química Universidad Nacional Autónoma de México Mexico City Mexico

4. Facultad de Medicina Universidad Nacional Autónoma de México Mexico City Mexico

Abstract

We report the characterization of the three‐dimensional structure of the CEMP1‐p1 peptide [MGTSSTDSQQAQHRRCSTSN: corresponding to residues 1–20 of the N‐terminus of cementum protein 1 (CEMP1)]. This peptide imitates the capacity of CEMP1 to stimulate hydroxyapatite (HA) crystal nucleation and growth, and promotes the differentiation of periodontal ligament cells into a cementoblastic phenotype. Additionally, in experimental models of critical‐sized calvarial defects in Wistar rats, CEMP1‐p1 has shown osteogenic properties that enhanced the physiological deposition and maturation of newly formed bone. In this work, studies of CEMP1‐p1 by circular dichroism (CD) and nuclear magnetic resonance (NMR) were performed in trifluoroethanol D2 (TFED2) and aqueous solution to determine the 3D structure of the peptide. Using the 3D model, experimental data from HA crystals formation and calcium fluorescence emission, we explain the biological mechanisms involved in CEMP1‐p1 activity to promote calcium recruitment and its affinity to HA crystals. This information is valuable because it proposes, for the first time, a plausible molecular mechanism during the mineralization process, from a specific cementum protein‐derived peptide.

Funder

Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México

Consejo Nacional de Ciencia y Tecnología, Paraguay

Publisher

Wiley

Subject

Organic Chemistry,Drug Discovery,Pharmacology,Molecular Biology,Molecular Medicine,General Medicine,Biochemistry,Structural Biology

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