Affiliation:
1. Institute of Forensic Medicine and Laboratory Medicine Jining Medical University Jining Shandong P. R. China
2. Jining Public Security Bureau Jining Shandong P. R. China
Abstract
AbstractShort tandem repeat (STR) automatic typing technology is extensively used in forensic laboratories with commercial kits, in rare cases genotyping misinterpretations or mislabeling may occur due to unexpected rare alleles. This study refers to the investigation of several rare alleles observed from routine cases. Besides cross‐kit verification with Goldeneye 25A (Beijing PeopleSpot Inc, China) and Huaxia platinum (Thermo Fisher Scientific, USA) kits, the next‐generation sequencing technology by MiSeq FGx System (Illumina, USA) was applied to further validation. To solve the inconsistent outcomes reached by the above mentioned approaches at D2S441 locus, single gene amplification, gene cloning, and genetic sequencing was also performed. As a result, five rare alleles were detected. Two novel alleles of allele 3 at the D13S317 locus and allele 5 at the D2S441 locus were found; three previously reported alleles of allele 9 at D1S1656 locus, allele 19 at Penta D locus, and allele 28 at D12S391 locus in STRBase were initially supplemented with sequence information. We, therefore, propose that such uncommon observations with rare events should be carefully investigated and interpreted.
Subject
Clinical Biochemistry,Biochemistry,Analytical Chemistry
Reference6 articles.
1. The validation of short tandem repeat (STR) loci for use in forensic casework
2. Short tandem repeat typing technologies used in human identity testing
3. Chelex 100 as a medium for simple extraction of DNA for PCR‐based typing from forensic material;Walsh PS;Biotechniques,1991
4. Nested polymerase chain reaction (PCR);Green MR;Cold Spring Harb Protoc,2019