Affiliation:
1. College of Acupuncture & Moxibustion and Tuina Beijing University of Chinese Medicine Beijing China
2. Clinic of Surgery of Traditional Chinese Medicine China‐Japan Friendship Hospital Beijing China
3. Department of Intelligent Manufacturing Petro‐Cyberworks Information Technology Company, Limited Beijing China
Abstract
AbstractObjectiveThis study aims to investigate the protective mechanism of moxibustion in combating atherosclerosis (AS).MethodsApolipoprotein E (ApoE)‐deficient mice, aged 8 weeks, were randomly assigned into four groups: the model group (n = 6), SC79 group (n = 6), moxibustion group (n = 6), and moxibustion+SC79 group (n = 6). All mice were fed with a high‐fat diet (HFD). Concurrently, 8‐week‐old C57BL/6 mice of the same genetic background were utilized as the control group (n = 6) and were given a regular diet. Macrophages were isolated via flow cytometry. The intracellular Ca2+ expression in macrophages was evaluated, and aortic plaques were quantitatively assessed through en face oil red O and Masson staining. The presence of macrophages and smooth muscle cells in AS plaques was determined by MAC‐3 and α‐smooth muscle actin (α‐SMA) immunohistochemistry. The relative fluorescence intensity of nuclear factor‐κB (NF‐κB) in macrophages was identified by immunofluorescence staining. The expressions of proteins related to the P2Y12/phosphatidylinositol 3‐hydroxy kinase (PI3K)/protein kinase B (AKT) signaling pathway were examined by Western blotting.ResultsMoxibustion reduced free Ca2+ expression in macrophage cytoplasm, inhibiting Ca2+ influx and oxidative stress. Significant reductions in atherosclerotic plaque formation and inflammation markers, including TNF‐α and IL‐1β, were noted in the moxibustion group. Moxibustion modulated the P2Y12/PI3K/AKT pathway, impacting various inflammatory and oxidative stress‐related proteins. Introduction of the AKT activator SC79 counteracted moxibustion's benefits, highlighting the P2Y12/PI3K/AKT pathway's central role.ConclusionMoxibustion, through the P2Y12/PI3K/AKT signaling pathway, can inhibit Ca2+ overload‐induced oxidative stress and inflammatory response, decrease macrophage infiltration, and increase the content of smooth muscle cells and collagen, thereby exerting a protective effect against AS.
Funder
National Natural Science Foundation of China
Subject
Health, Toxicology and Mutagenesis,Management, Monitoring, Policy and Law,Toxicology,General Medicine
Reference22 articles.
1. Organization WH.Global Health Estimates: Life Expectancy and Leading Causes of Death and Disability.2023.https://www.who.int/data/gho/data/themes/mortality-and-global-health-estimates
2. Addressing the global burden of cardiovascular diseases; need for scalable and sustainable frameworks. Global;Mendis S;Heart,2022
3. Acute coronary syndromes
4. Inhibition of macrophage-derived foam cells by Adipsin attenuates progression of atherosclerosis
5. Tuning macrophages for atherosclerosis treatment. Regenerative;Fang F;Biomaterials,2023
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