Affiliation:
1. School of Agriculture Food and Ecosystem Faculty of Sciences The University of Melbourne Parkville 3010, VIC Australia
2. Algal Processing Group Department of Chemical Engineering The University of Melbourne Parkville Victoria 3010 Australia
Abstract
AbstractThis study compared free and bound phenolic compounds in various marine microalgae species. It assessed total phenolic content (TPC), total flavonoid content (TFC) and total condensed tannin content (TCT) and their antioxidant capacities using 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) assay, 2,2’‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS⋅+) radical cation‐based assay and ferric ion reducing antioxidant power assay. Liquid chromatography‐mass spectrometry (LC–MS) was also employed to characterize the phenolic profiling. Results showed that free phenolic compounds ranged from 1.83–6.45 mg GAE/g d. w., while bound phenolic compounds ranged from 4.03–26.03 mg GAE/g d. w., indicating significant differences. These variations were consistent across assays, highlining unique profiles in different species. A total 10 phenolics were found in these seven microalgae, including 1 phenolic acid, 6 flavonoids, 1 other polyphenol and 2 lignans. 4′‐O‐methyl‐(−)‐epigallocatechin 7‐O‐glucuronide and chrysoeriol 7‐O‐glucoside in microalgae were firstly reported in microalgal samples. These findings have implications for future applications in industries.
Funder
Australian Research Council
University of Melbourne
Subject
Molecular Biology,Molecular Medicine,General Chemistry,Biochemistry,General Medicine,Bioengineering