Affiliation:
1. Engineering Research Center for Medicine Ministry of Education Harbin University of Commerce Harbin 150076 People's Republic of China
2. Technology Innovation Center for Exploitation of Marine Biological Resources Third Institute of Oceanography Ministry of Natural Resources Xiamen 361005 People's Republic of China
Abstract
AbstractOne novel bisabolane‐derived sesquiterpenoid retrobisabolane A (1), featuring a methyl group location at the C‐4 position instead of C‐3 in the bisabolanes, and a known ester‐substituted eremophilane‐type sesquiterpenoid cryptosphaerolide (2), along with three known indole alkaloids (3–5) were discovered from the fermented cultures of a deep‐sea‐derived fungus Retroconis fusiformis MCCC 3A00792. The planar structure of new compound 1 was determined by extensive analysis of the NMR and HRESIMS spectra. The relative and absolute configurations of 1 were resolved by the coupling constant (J), calculation of ECD and NMR spectra, and the DP4+ probability analysis of the 1H and 13C NMR data. Interestingly, retrobisabolane A was the new subclass of bisabolanes bearing a methyl group linkage at C‐4 instead of C‐3 position. Three human cancer cell lines (Hela, AGS, and BIU‐87) were subjected to evaluate the cytotoxic activities of compounds 1–5. As a result, compound 2 exhibited significant inhibitory activities against three cell lines with IC50 values ranging from 9.95 to 18.77 μM.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China