Transcriptomics Analysis of the Immune Effects of Okadaic Acid on Caco‐2 Cells

Abstract

AbstractOkadaic Acid, a type of diarrhetic shellfish poison, is widely distributed and harmful, causing symptoms such as diarrhea, vomiting, and more in humans. Recent studies have demonstrated that OA can lead to various toxicities such as cytotoxicity, neurotoxicity, embryotoxicity, and hepatotoxicity. In order to investigate the immunotoxicity of OA on intestinal cells, a transcriptome analysis was conducted to compare the differences in the Caco‐2 cell transcriptional group before and after administration. The CCK‐8 experiment demonstrated that OA had a detrimental effect on the activity of Caco‐2 cells, with an IC50 value of 33.98 nM. Transcriptome data revealed changes in immune‐related genes between the experimental and control groups, including inflammatory factors, heat shock proteins, and zinc finger proteins. The analysis of the results suggests that OA can induce the production of inflammatory factors and apoptosis in cells, and may also affect cell ferroptosis. These findings indicate that OA has a significant impact on intestinal immunity, providing valuable insights for the study of immune toxicity associated with OA.