Affiliation:
1. State Key Laboratory of Fine Chemicals Dalian R&D Center for Stem Cell and Tissue Engineering Dalian University of Technology Dalian China
2. Zhengzhou Institute of Emerging Industrial Technology Zhengzhou China
3. Institute for Biomedical Materials and Devices Faculty of Science University of Technology Sydney NSW 2007 Australia
Abstract
AbstractFlavonoids, known for their abundance in Eucommia ulmoides pollen, possess diverse biological functions, including antioxidants, antibacterial agents, and anti‐tumor properties. This study aims to establish effective parameters for flavonoid extraction from Eucommia ulmoides pollen using a microwave‐assisted method, characterize the flavonoid composition of the extracted material, and explore its biological activities. Building upon the initial results from single‐factor experiments, response surface methodology was employed to optimize the extraction parameters. The inhibitory effect of human breast cancer cells (MCF‐7) was evaluated by CCK assay and Live/dead staining. Simultaneously, the extract‘s scavenging ability against DPPH free radicals and its antibacterial properties against Escherichia coli and Staphylococcus aureus were investigated. The results demonstrated that the flavonoid yield reached 3.28 g per 100 g of pollen, closely aligning with the predicted value. The IC50 for flavonoid‐mediated DPPH radical scavenging was 0.04 mg/mL. The extract exhibited a robust inhibitory effect on both Escherichia coli and Staphylococcus aureus. Concurrently, the extract displayed a significant inhibitory effect on the growth and proliferation of MCF‐7 cells in a dose‐dependent and time‐dependent manner. In addition, six kinds of flavonoids have been identified by UPLC‐TOF‐MS/MS technology, providing further support to the study on the anti‐oxidation and anti‐tumor mechanism of Eucommia ulmoides pollen extracts.
Funder
National Natural Science Foundation of China
Fok Ying Tung Education Foundation
Fundamental Research Funds for the Central Universities
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献