A high cell density transient transfection system for therapeutic protein expression based on a CHO GS-knockout cell line: Process development and product quality assessment-Reference-Cited by-同舟云学术

A high cell density transient transfection system for therapeutic protein expression based on a CHO GS-knockout cell line: Process development and product quality assessment

Published:2015-03-16 Issue:5 Volume:112 Page:977-986
ISSN:0006-3592
Container-title:Biotechnology and Bioengineering
language:en
Short-container-title:Biotechnol. Bioeng.

Author:

Rajendra Yashas¹,Hougland Maria D.¹,Alam Riazul¹,Morehead Teresa A.¹,Barnard Gavin C.¹

Affiliation:

1. Biotechnology Discovery Research; Lilly Research Laboratories; Eli Lilly and Company; Lilly Corporate Center; Indianapolis 46285 Indiana

Publisher

Wiley

Subject

Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Reference29 articles.

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4. High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI;Backliwal;Biotechnol Bioeng,2008b

5. Recombinant protein production by large-scale transient gene expression in mammalian cells: State of the art and future perspectives;Baldi;Biotechnol Lett,2007

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