Elevated Carbon Dioxide Exposure Alters Intracellular pH and Energy Charge in Avocado Fruit Tissue

Abstract

Changes in cytosolic and vacuolar pH, ATP, ADP, and the ATP : ADP ratio were measured in whole fruit or mesocarp disks of avocado [Persea americana (Mill.) cv. Hass] during brief exposures to elevated CO2. Intact climacteric fruit exposed to air (21% O2), 20% CO2 (17% O2, balance N2), or 40% CO2 (13% O2, balance N2) had cytosolic pH values of 7.0, 6.6, and 6.4, respectively, while mesocarp disks had cytosolic pH values of 6.9, 6.7, and 6.4, respectively. The ß-ATP levels of intact climacteric fruit exposed to 20% CO2 or 40% CO2 for 2 h were reduced by 25% or 43%, respectively, relative to air-exposed fruit. HPLC analysis of nucleotide phosphates from preclimacteric avocados revealed that ATP levels and the ATP : ADP ratio increased in 40% compared to the air-stored fruit. However, 1 day after transfer to air, the effects of elevated CO2 had dissipated. These modifications in cellular state could alter the activity of respiratory enzymes in fruit exposed to elevated CO2 atmospheres.