Clip-and-wash Method of Emasculation for Lettuce

Abstract

Complete (100%) hybridization in lettuce (Lactuca sativa L.) was accomplished consistently using the clip-and-wash method of emasculation. The clip-and-wash method is a combination of washing and clipping, two previously described procedures for pollen removal. The wash and the clip methods produced 98% and 95% hybridization, respectively. The method is quick and easy and eliminates inadvertent self pollination; with it one can produce the many hybrid seeds necessary for backcross and F1 genetic analysis in lettuce.