Phenotypic and molecular determination of anthracnose disease resistance in Lake Van Basin’s bean genotypes (Phaseolus vulgaris L.)

Abstract

In this study, the resistance levels to anthracnose disease [Colletotrichum lindemuthianum (Sacc. and Magnus) Lambs. Scrib.] of 92 bean genotypes collected from different parts of the Lake Van Basin were investigated by artificial inoculation and molecular markers. The resistance levels of bean genotypes to the isolate 11# of anthracnose disease were determined by classical inoculation method in a climate chamber condition, and the presence of resistance gene related markers in bean genotypes was determined by using four SCAR primers [SAS13 (950 bp, Co-42), SC08 (910 bp, Co-4), SF10 (1072 bp, Co-10), SZ04 (567 bp, Co-6)] and one RAPD [OA181500 (1500 bp, Co-15)] primer. In the artificial inoculation, the bean genotypes were evaluated according to the 0-9 scale, and the four of them having a value between 0 and 3 were found as resistant to this isolate of anthracnose, but the rest of them having a value between 4 and 9 were determined as sensitive. With molecular markers, it was found that the 82 bean genotypes had the resistant Co-42 allele; the 54 bean genotypes had the resistant Co-4 allele; the 6 bean genotypes had the resistant Co-10 allele; the 36 bean genotypes had the resistant Co-6 allele; and the 15 bean genotypes had the resistant Co-15 allele. The only bean genotype having all resistance markers was the genotype G81, whereas the bean genotypes G27, G28, G40, G76, and G86 had no resistance associated molecular markers.