Cryoprotective Activity of Glycerol and Ethylene Glycol: Dynamics with Canine Sperm Integrity

Abstract

Background: Different cryoprotectants had been used for safe gaurding the post thaw Canine sperm cell motility. Glycerol and ethylene glycol may be used as cryoprotectants. However, scanty reports are available on use of different concentrations of glycerol, ethylene glycol and the combination of glycerol and ethylene glycol as cryoprotectants for canine spermatatozoa. Methods: In present study, the semen samples were collected from 4 different adult spitz male dogs. The diluted semen was divided into four aliquots, Group I: 8% glycerol (control); Group II: 4% glycerol; Group III: 5% ethylene glycol; Group IV: 4% glycerol + 4% ethylene glycol. Result: The result showed that post-thaw sperm motility in canine semen with 5% ethylene glycol was recorded as 36.83±1.26%. For Group I (8% glycerol) and Group II (4% glycerol), the same was 34.25±0.95% and 26.50±0.81%, respectively whereas Group IV (4% glycerol + 4% ethylene glycol) it was 29.25%. However, there was no significant correlationship between abnormal sperm count and live spermatozoa; abnormal sperm count and dead spermatozoa; dead spermatozoa and DNA integrity and in between HOST (hypo-osmotic swelling test) and acrosomal integrity of spermatozoa.