Chemical Composition, Antioxidant Potential, and Antibacterial Activity of Essential Oil Cones of TunisianCupressus sempervirens

Author:

Ben Nouri Aicha1,Dhifi Wissal2,Bellili Sana1,Ghazghazi Hanene3,Aouadhi Chedia4,Chérif Ameur1,Hammami Mohamed56,Mnif Wissem17

Affiliation:

1. LR11-ES31 Biotechnologie et Valorisation des Bio-Géo Ressources, Institut Supérieur de Biotechnologie de Sidi Thabet, Université de la Manouba, BiotechPole de Sidi Thabet, 2020 Sidi Thabet, Tunisia

2. UR Ecophysiologie Environnementale et Procédés Agroalimentaires, Université de la Manouba, BiotechPole de Sidi Thabet, 2020 Sidi Thabet, Tunisia

3. Laboratory of Management and Valorization of Forest Resources, National Research Institute of Rural Engineering, Water and Forestry (INRGREF), 1002 Tunis, Tunisia

4. Laboratoire d’Epidémiologie et Microbiologie Vétérinaire, Groupes de Bactériologie et Développement Biotechnologique, Institut Pasteur de Tunis, Université El Manar, 1002 Tunis, Tunisia

5. USCR de Spectrométrie de Masse, Laboratoire de Biochimie, Faculté de Médecine, 5019 Monastir, Tunisia

6. Institut National de Recherche et d’Analyses Physicochimique (INRAP), Université de la Manouba, BiotechPole de Sidi Thabet, 2020 Sidi Thabet, Tunisia

7. Faculty of Sciences and Arts in Balgarn, Bisha University, P.O. Box 60, Balgarn, Sabt Al Alaya 61985, Saudi Arabia

Abstract

The extraction yield of the essential oil (EO) extracted by hydrodistillation from the cones of TunisianCupressus sempervirensL. was of 0.518%. The chemical composition was analyzed by GC-MS. Results showed that this essential oil was mainly composed of monoterpene hydrocarbons (65%) withα-pinene as the major constituent (47.51%). Its antioxidant activity was ascertained by evaluating the total antioxidant capacity and also by evaluating its inhibitory effect against DPPH and ABTS radicals. In addition, it showed a strong antioxidant power against the DPPH (IC50= 151 µg/mL) and ABTS (IC50= 176.454 µg/mL) radicals scavenging. Moreover, its antibacterial activity was tested against different species of pathogenic bacteria (three Gram-positive and eight Gram-negative bacteria). The bacterial strains susceptible to the evaluated oil wereBacillus subtilis,Escherichia coli,Klebsiella oxytoca,Morganella morganii,Shigella, andVibrio cholerae.

Funder

Tunisian Ministry of Higher Education and Scientific Research

Publisher

Hindawi Limited

Subject

General Chemistry

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